smart seq (TaKaRa)

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TaKaRa smart seq
Smart Seq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smart+seq+stranded+kit/SMART-Seq+Stranded+Kit/pm42810342-370-8-10
Average 96 stars, based on 343 article reviews

smart seq - by Bioz Stars, 2026-10

96/100 stars

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cDNA Synthesis:

Article Title: Juvenile social isolation induces gene expression alterations and histone modification dysregulations in nucleus accumbens (NAc) neurons
Article Snippet: .. After removing the supernatant, nuclei were resuspended in 3.5 μl of water and transferred to a new tube for cDNA synthesis and library preparation using the SMART-seq Stranded Kit (Takara, #634447) according to the manufacturer’s protocol. .. Library concentrations were assessed using the TapeStation system (Agilent), and sequencing was performed on the DNB-seq platform (MGI) to obtain 50-base single-end reads.

RNA sequencing:

Article Title: Intraductal Papillary Squamous Neoplasm (IPSN) of the Pancreas: Histologic and Molecular Characterization of a Novel and Distinct Intraductal Cancer Precursor.
Article Snippet: .. RNAseq was performed using SMART-Seq Stranded kit (Takara Bio, Shiga, Japan), according to the manufacturer’s instructions. .. The RNA libraries were sequenced on NovaSeq 6000 (Illumina, San Diego, California).

RNA Sequencing:

Article Title: Disrupted epigenetic regulation in human senile lentigines revealed by characterizing gene expression and DNA methylation alterations.
Article Snippet: .. RNA-seq libraries were constructed with 5 ng of total RNA using SMART-Seq Stranded Kit (TaKaRa, Kusatsu, Japan), in accordance with the manufacturer’s manual. ..

Article Title: Tofogliflozin alters amino acid metabolism in gut microbiota linked to hepatic transcriptomic signatures in MASLD.
Article Snippet: Background A deeper understanding of the relationship between dysbiotic gut microbiota and liver tissue-level molecular and histopathological phenotypes in metabolic dysfunction-associated steatotic liver disease (MASLD) remains needed.. We aimed to characterize the associations between gut microbial metabolic functions and treatment responses in participants with MASLD.. Methods We performed a prespecified sub-analysis of a randomized controlled trial comparing the sodium–glucose cotransporter 2 inhibitor (SGLT2i) tofogliflozin and the sulfonylurea (SU) glimepiride in participants with MASLD and type 2 diabetes (ClinicalTrials.gov NCT02649465).

Construct:

other:

Article Title: Single-cell atlas reveals age-related cellular shifts underlying fibrosis in murine synovium
Article Snippet: Libraries were prepared with the SMART-Seq Stranded kit (Takara Bio) and were sequenced with a SP50 paired end flow cell on a NovaSeq 6000 (Illumina).

Sequencing:

Article Title: Tumor-associated calcium signal transducer 2 expression as a promising therapeutic target in esophageal squamous cell carcinoma: a combined the Cancer Genome Atlas and an institutional cohort.
Article Snippet: Briefly, total RNA was extracted from two FFPE tissue slices using an miRNeasy FFPE kit (Qiagen) [20]. .. Whole-transcriptome sequencing was performed using the SMART-Seq Stranded kit (Takara Bio Europe) for library preparation and NextSeq 500, single end 75 bp (Illumina) for sequencing [21]. ..

Review